Development of a Microfluidic Paper-Based Immunoassay for Rapid Detection of Allergic Protein in Foods

May 27, 2021

Meals allergy impacts as much as 10% of the inhabitants, inflicting average to extreme systemic signs and even loss of life. Detecting allergens in meals is probably the most crucial and efficient measure to scale back meals allergy incidents, however probably the most generally used kits, lateral move immunoassay (LFI), are nonetheless comparatively excessive in value for the meals business. Microfluidic paper-based analytical units (μPADs) reveal the potential to handle this problem by substituting the a number of elements/supplies in LFI with solely (cellulose) paper because the platform.

This examine experiences the event of a single-piece lateral move μPAD and demonstrates its functionality of detecting allergic protein in varied meals samples inside a 15 min together with pattern preparation. A confined reagent deposition on the testing zone of the patterned paper was achieved by blade reducing. Floor modification of the cellulose was optimized to allow the whole launch of recognizing conjugates and elimination of background noise. The geometry of patterns was optimized to satisfy the liquid pattern’s requirement in move price.

This LFI-format μPAD can detect as little as 1 ppm ovalbumin in commonplace answer, 0.01% (w/w) egg white protein in spiked cake combine, and the egg content material in different industrial meals merchandise. A lot easier fabrication procedures and a decrease materials value are required by the μPAD than that by typical LFIs. With the potential to scale up, this examine offers another meals monitoring choice to many sectors all through the agri-food chain and contributes to enhancing meals security and public well being.

A Systematic Evaluate of Meals Allergy: Nanobiosensor and Meals Allergen Detection

A number of people will expertise unintentional publicity to an allergen. On this sense, the business has invested within the processes of eradicating allergenic compounds in meals. Nonetheless, unintentional publicity to allergenic proteins may end up from allergenic substances not specified on labels. Evaluation of allergenic meals is concerned in strategies primarily based on immunological, genetic, and mass spectrometry.

The normal strategies have some limitations, resembling excessive value. Lately, biosensor and nanoparticles mixed have emerged as delicate, selective, low-cost, and time-consuming methods that may exchange traditional methods. However, every nanomaterial has proven a distinct potential to particular allergens or courses. This evaluation used Most well-liked Reporting Objects for Systematic Critiques and the Meta-Evaluation pointers (PRISMA) to method these points.

A complete of 104 articles had been retrieved from a standardized search on three databases (PubMed, Scopus and Internet of Science). The systematic evaluation article is organized by the class of allergen detection and nanoparticle detection. This evaluation addresses the related biosensors and nanoparticles as gold, carbon, graphene, quantum dots to allergen protein detection. Among the many chosen articles it was doable to note a larger potential utility on the allergic proteins Ah, in peanuts and gold nanoparticle-base as a biosensor. We envision that in our evaluation, the affiliation between biosensor and nanoparticles has proven promise within the evaluation of allergenic proteins current in several meals samples.

Pollen, a significant causal agent of respiratory allergy, is principally affected by climate situations. In Korea, pollen and climate knowledge are collected by the nationwide remark community. Forecast fashions and operational providers are developed and supplied primarily based on the nationwide pollen knowledge base. Utilizing the pollen danger forecast data will assist sufferers with respiratory allergy to enhance their lives. Modifications in temperature and CO2 focus by local weather change have an effect on the expansion of crops and their capability of manufacturing extra allergenic pollens, which needs to be thought-about in making the long run technique on treating allergy sufferers.

Development of a Microfluidic Paper-Based Immunoassay for Rapid Detection of Allergic Protein in Foods

Improvement and Validation of a Deep Studying Mannequin for Detection of Allergic Reactions Utilizing Security Occasion Reviews Throughout Hospitals

This cross-sectional examine analyzed hospital security experiences filed between Might 2004 and January 2019 at Brigham and Girls’s Hospital and between April 2006 and June 2018 at Massachusetts Common Hospital in Boston. Coaching and validating a deep studying mannequin concerned extracting security experiences utilizing 101 expert-curated key phrases from Massachusetts Common Hospital (knowledge set I).
The mannequin was then evaluated on three knowledge units: experiences with out key phrases (knowledge set II), experiences from a distinct time-frame (knowledge set III), and experiences from a distinct hospital (Brigham and Girls’s Hospital; knowledge set IV). Statistical analyses had been carried out between March 1, 2019, and July 18, 2020.
The world underneath the receiver working attribute curve and space underneath the precision-recall curve had been used on knowledge set I. The precision at top-k was used on knowledge units II to IV. A complete of 299 028 security experiences with 172 854 sufferers had been included. Of those sufferers, 86 544 had been ladies (50.1%) and the median (interquartile vary [IQR]) age was 59.7 (43.8-71.6) years. The deep studying mannequin achieved an space underneath the receiver working attribute curve of 0.979 (95% CI, 0.973-0.985) and an space underneath the precision-recall curve of 0.809 (95% CI, 0.773-0.845).

Peanut oil
GK9829-100ML 100 ml
EUR 54

Peanut oil
GK9829-1L 1 l
EUR 130

Peanut oil
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Peanut oil
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EUR 90

Peanut ELISA Kit
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EUR 845
Description: Enzyme Immunoassay for the Quantitative Determination of Peanut in Food

Peanut procyanidin A
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Peanut procyanidin E
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Peanut Protein Antibody
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Peanut Protein Antibody
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Description: Peanut Protein Monoclonal

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Description: Peanut Protein Monoclonal

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Description: Peanut Protein Monoclonal

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EUR 300
Description: Peanut Protein Monoclonal

Peanut Protein Antibody
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Description: Peanut Protein Polyclonal

Cortisol Saliva Kit
55R-2266 1 Kit
EUR 435
Description: Cortisol Saliva ELISA Kit

Xylene - Peanut Oil Solution
XPO125 125 ml
EUR 72

Xylene - Peanut Oil Solution
XPO500 500 ml
EUR 104

Xylene - Peanut Oil Solution
XPO999 1000 ml
EUR 136

Peanut Oil vaccine adjuvant
AV-5010-50 50 ml
EUR 225

EnzyChrom Peanut ELISA Kit
EPNT-100 100
EUR 599
Description: Determination of peanut in food and beverage samples

IgA Saliva ELISA Kit
DEIA-S216 96T
EUR 709
Description: Immunoenzymatic colorimetric method for determination of IgA in saliva. Salivary IgA ELISA is intended for laboratory use only.

Saliva DNA Purification Kit
K1468-250 250 Preps
EUR 551

Saliva DNA Purification Kit
K1468-50 50 Preps
EUR 318

Alpha-Amylase, Human Saliva
P1455-1
EUR 207

Saliva DNA Isolation Kit
K5011050 1 kit
EUR 256

Chicken egg ovalbumin protein (ELISA Kit, antigen, allergy grade)
OVA15-N-1000 1 g
EUR 408

Peanut agglutinin,PNA ELISA Kit
QY-E50016 96T
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Peanut agglutinin,PNA ELISA Kit
CN-01043P1 96T
EUR 434

Peanut agglutinin,PNA ELISA Kit
CN-01043P2 48T
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Lyophilized Exosome Standard (Human Saliva)
ESL-27 30 µgx6 vials
EUR 3023
Description: Lyophilized exosomes can be used as control standards for FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples.

Human (Saliva) Amylase alpha Enzyme
abx070002-1000Units 1000 Units
EUR 398

ExoFACS? Kit for Saliva Exosomes
K1234-20
EUR 794

Cortisol (Saliva) Human ELISA Kit
K7434-100
EUR 561

ExoStd? Saliva Fluorescent Exosome Standard
M1076-100
EUR 756

CF488A Arachis hypogaes Lectin PNA (Peanut)
29060 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF568 Arachis hypogaes Lectin PNA (Peanut)
29061 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF594 Arachis hypogaes Lectin PNA (Peanut)
29062 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF640R Arachis hypogaes Lectin PNA (Peanut)
29063 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

Human Saliva Peroxidase(SP)ELISA Kit
CSB-E13593h-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Saliva Peroxidase (SP) in samples from serum, plasma, salvia, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Saliva Peroxidase(SP)ELISA Kit
1-CSB-E13593h
  • EUR 703.00
  • EUR 4843.00
  • EUR 2570.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Saliva Peroxidase(SP) in samples from serum, plasma, salvia, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Cortisol in Saliva Elisa Kit (RUO)
55R-RE52061 96 wells
EUR 358
Description: ELISA kit for detection of Free Cortisol in Saliva in the research laboratory

Aflatoxins B1 ELISA test kit (Crops: corn, rice, peanut, peanut butter, feed), 96 tests, quantitative
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ELISA kit for Human Saliva Peroxidase (SP)
KTE60553-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Human Saliva Peroxidase (SP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Saliva Peroxidase (SP)
KTE60553-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Saliva Peroxidase (SP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Saliva Peroxidase (SP)
KTE60553-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Human Saliva Peroxidase (SP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Purified Exosomes from Human Saliva (Healthy Donors)
EXOP-510A-1 >25ug
EUR 419

Easy-Exo Saliva Exosome Isolation Kit, 95% pure
P530 - Ask for price

Easy-Exo Saliva Exosome Isolation Kit, 95% pure
P530S - Ask for price

Human Progesterone (saliva) ELISA Kit, 96 tests, Quantitative
1855 1 kit
EUR 590

Human Androstenedione (saliva) ELISA Kit, 96 tests, Quantitative
1915 1 kit
EUR 590

Human Cortisol (saliva) ELISA Kit, 96 tests, Quantitative
3500 1 kit
EUR 529

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)
M1010-100-T
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)
M1011-100-W
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)
M1012-100-B
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)
M1013-100-T
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)
M1014-100-W
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)
M1015-100-B
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Coronavirus (SARS-Cov-2) Antigen Rapid Test Device (Saliva)
IOV87952 20T/kit
EUR 39
Description: Coronavirus (SARS-Cov-2) Antigen Rapid Test Device (Saliva) is an in vitro diagnostic test for the qualitative detection of novel coronavirus antigens in human saliva, using the rapid immunochromatographic method. The identification is based on the monoclonal antibodies specific for the novel coronvirus antigen. It will provide information for clinical doctors to prescribe correct medications.

Mouse Anti-Peanut allergen Ara H1 monoclonal antibody, clone Arah1D
HMABPY116D-100ug 100 ug
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Mouse Anti-Peanut allergen Ara H1 monoclonal antibody, clone Arah1D
HMABPY116D-1mg 1 mg
EUR 1313

Mouse Anti-Peanut allergen Ara H2 monoclonal antibody, clone Arah2D
HMABPY117D-100ug 100 ug
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Mouse Anti-Peanut allergen Ara H2 monoclonal antibody, clone Arah2D
HMABPY117D-1mg 1 mg
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ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 2 vials)
M1046-2
EUR 604

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 4 vials)
M1046-4
EUR 914

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 6 vials)
M1046-6
EUR 1306

ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 2 vials)
M1047-2
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ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 4 vials)
M1047-4
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ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 6 vials)
M1047-6
EUR 1621

Oil Adjuvant Combo Pak-1 (Contains 10 ml Peanut Oil (#AV-5010-10); Mineral oil (#AV-5020-10) and 1 g Mannide monooleate (#AV-5030-1)
AV-5000-PK-1 1 pk
EUR 164
The mannequin achieved precisions on the high 100 model-identified instances of 0.930 in knowledge set II, 0.960 in knowledge set III, and 0.990 in knowledge set IV. In contrast with the keyword-search method, the deep studying mannequin lowered the variety of instances for handbook evaluation by 63.8% and recognized 24.2% extra instances of confirmed allergic reactions. The mannequin highlighted vital phrases (eg, rash, hives, and Benadryl) in prediction and prolonged the record of expert-curated key phrases by means of an consideration layer.