Development of a Microfluidic Paper-Based Immunoassay for Rapid Detection of Allergic Protein in Foods

Meals allergy impacts as much as 10% of the inhabitants, inflicting average to extreme systemic signs and even loss of life. Detecting allergens in meals is probably the most crucial and efficient measure to scale back meals allergy incidents, however probably the most generally used kits, lateral move immunoassay (LFI), are nonetheless comparatively excessive in value for the meals business. Microfluidic paper-based analytical units (μPADs) reveal the potential to handle this problem by substituting the a number of elements/supplies in LFI with solely (cellulose) paper because the platform.

This examine experiences the event of a single-piece lateral move μPAD and demonstrates its functionality of detecting allergic protein in varied meals samples inside a 15 min together with pattern preparation. A confined reagent deposition on the testing zone of the patterned paper was achieved by blade reducing. Floor modification of the cellulose was optimized to allow the whole launch of recognizing conjugates and elimination of background noise. The geometry of patterns was optimized to satisfy the liquid pattern’s requirement in move price.

This LFI-format μPAD can detect as little as 1 ppm ovalbumin in commonplace answer, 0.01% (w/w) egg white protein in spiked cake combine, and the egg content material in different industrial meals merchandise. A lot easier fabrication procedures and a decrease materials value are required by the μPAD than that by typical LFIs. With the potential to scale up, this examine offers another meals monitoring choice to many sectors all through the agri-food chain and contributes to enhancing meals security and public well being.

A Systematic Evaluate of Meals Allergy: Nanobiosensor and Meals Allergen Detection

A number of people will expertise unintentional publicity to an allergen. On this sense, the business has invested within the processes of eradicating allergenic compounds in meals. Nonetheless, unintentional publicity to allergenic proteins may end up from allergenic substances not specified on labels. Evaluation of allergenic meals is concerned in strategies primarily based on immunological, genetic, and mass spectrometry.

The normal strategies have some limitations, resembling excessive value. Lately, biosensor and nanoparticles mixed have emerged as delicate, selective, low-cost, and time-consuming methods that may exchange traditional methods. However, every nanomaterial has proven a distinct potential to particular allergens or courses. This evaluation used Most well-liked Reporting Objects for Systematic Critiques and the Meta-Evaluation pointers (PRISMA) to method these points.

A complete of 104 articles had been retrieved from a standardized search on three databases (PubMed, Scopus and Internet of Science). The systematic evaluation article is organized by the class of allergen detection and nanoparticle detection. This evaluation addresses the related biosensors and nanoparticles as gold, carbon, graphene, quantum dots to allergen protein detection. Among the many chosen articles it was doable to note a larger potential utility on the allergic proteins Ah, in peanuts and gold nanoparticle-base as a biosensor. We envision that in our evaluation, the affiliation between biosensor and nanoparticles has proven promise within the evaluation of allergenic proteins current in several meals samples.

Pollen, a significant causal agent of respiratory allergy, is principally affected by climate situations. In Korea, pollen and climate knowledge are collected by the nationwide remark community. Forecast fashions and operational providers are developed and supplied primarily based on the nationwide pollen knowledge base. Utilizing the pollen danger forecast data will assist sufferers with respiratory allergy to enhance their lives. Modifications in temperature and CO2 focus by local weather change have an effect on the expansion of crops and their capability of manufacturing extra allergenic pollens, which needs to be thought-about in making the long run technique on treating allergy sufferers.

Development of a Microfluidic Paper-Based Immunoassay for Rapid Detection of Allergic Protein in Foods

Improvement and Validation of a Deep Studying Mannequin for Detection of Allergic Reactions Utilizing Security Occasion Reviews Throughout Hospitals

This cross-sectional examine analyzed hospital security experiences filed between Might 2004 and January 2019 at Brigham and Girls’s Hospital and between April 2006 and June 2018 at Massachusetts Common Hospital in Boston. Coaching and validating a deep studying mannequin concerned extracting security experiences utilizing 101 expert-curated key phrases from Massachusetts Common Hospital (knowledge set I).
The mannequin was then evaluated on three knowledge units: experiences with out key phrases (knowledge set II), experiences from a distinct time-frame (knowledge set III), and experiences from a distinct hospital (Brigham and Girls’s Hospital; knowledge set IV). Statistical analyses had been carried out between March 1, 2019, and July 18, 2020.
The world underneath the receiver working attribute curve and space underneath the precision-recall curve had been used on knowledge set I. The precision at top-k was used on knowledge units II to IV. A complete of 299 028 security experiences with 172 854 sufferers had been included. Of those sufferers, 86 544 had been ladies (50.1%) and the median (interquartile vary [IQR]) age was 59.7 (43.8-71.6) years. The deep studying mannequin achieved an space underneath the receiver working attribute curve of 0.979 (95% CI, 0.973-0.985) and an space underneath the precision-recall curve of 0.809 (95% CI, 0.773-0.845).

Single Donor Human Peanut Allergy Saliva

IRHUSLSPAL250UL each
EUR 369
Description: Single Donor Human Peanut Allergy Saliva

Peanut Allergen Detection Kit (PCR)

abx092288-100l 100 µl
EUR 775

Peanut Allergen Detection Kit (PCR)

abx092288-1ml 1 ml Ask for price

Peanut Allergen Detection Kit (PCR)

abx092288-200l 200 µl
EUR 1437.5

OPCA04608-1MG - Allergen Ara h 1, clone P17 Recombinant Protein (Peanut)

OPCA04608-1MG 1mg
EUR 1977

OPCA05378-20UG - Allergen Ara h 1, clone P41B Recombinant Protein (Peanut)

OPCA05378-20UG 20ug
EUR 863

OPCA04608-100UG - Allergen Ara h 1, clone P17 Recombinant Protein (Peanut)

OPCA04608-100UG 100ug
EUR 586

OPCA04608-20UG - Allergen Ara h 1, clone P17 Recombinant Protein (Peanut)

OPCA04608-20UG 20ug
EUR 400

OPCA05378-100UG - Allergen Ara h 1, clone P41B Recombinant Protein (Peanut)

OPCA05378-100UG 100ug
EUR 988

Peanut

DEPEAE01 96
EUR 265

Peanut Oil

25617-25 500G
EUR 16.1

Peanut oil

GK9829-1 1
EUR 98.8

Peanut oil

GK9829-100 100
EUR 23.8

Peanut oil

GK9829-100ML 100 ml
EUR 64.8

Peanut oil

GK9829-1L 1 l
EUR 156

Peanut oil

GK9829-250 250
EUR 39.4

Peanut oil

GK9829-250ML 250 ml
EUR 84

Peanut oil

GK9829-500 500
EUR 59.3

Peanut oil

GK9829-500ML 500 ml
EUR 108

Peanut Oil

P800200 10ml
EUR 87
Description: 8002-03-7

Peanut agglutinin

AT047 1mg
EUR 1134

Peanut agglutinin

AG047 1 mg
EUR 627.6

Peanut ELISA Kit

DEIA296 96T
EUR 1014
Description: Enzyme Immunoassay for the Quantitative Determination of Peanut in Food

Crude Peanut Extract

3069 10 mg
EUR 154
Description: Allergy, Immunology

Peanut procyanidin A

TBW01449 unit Ask for price

Peanut procyanidin D

TBW01450 unit Ask for price

Peanut procyanidin E

TBW01658 unit Ask for price

Peanut Protein Antibody

10-2833 1 mg
EUR 345
Description: Peanut Protein Monoclonal

Peanut Protein Antibody

10-2834 1 mg
EUR 345
Description: Peanut Protein Monoclonal

Peanut Protein Antibody

10-2835 1 mg
EUR 345
Description: Peanut Protein Monoclonal

Peanut Protein Antibody

10-2836 1 mg
EUR 345
Description: Peanut Protein Monoclonal

Peanut Protein Antibody

10-2837 1 mg
EUR 345
Description: Peanut Protein Monoclonal

Peanut Protein Antibody

10-2838 1 mg
EUR 345
Description: Peanut Protein Monoclonal

Peanut Protein Antibody

20-1739 1 mg
EUR 345
Description: Peanut Protein Polyclonal

EnzyChrom Peanut ELISA Kit

EPNT-100 100
EUR 718.8
Description: Determination of peanut in food and beverage samples

Xylene - Peanut Oil Solution

XPO125 125 ml
EUR 18.01

Xylene - Peanut Oil Solution

XPO500 500 ml
EUR 43.71

Xylene - Peanut Oil Solution

XPO999 1000 ml
EUR 69.43

Peanut Oil vaccine adjuvant

AV-5010-50 50 ml
EUR 270

Peanut lectin (PNA) ELISA Kit

SL0076PI - Ask for price

Peanut agglutinin,PNA ELISA Kit

CN-01043P1 96T
EUR 520.8

Peanut agglutinin,PNA ELISA Kit

CN-01043P2 48T
EUR 340.8

Peanut agglutinin,PNA ELISA Kit

QY-E50016 96T
EUR 495.6

Peanut agglutinin,PNA ELISA Kit

SL0001PI -
EUR 528

Aflatoxins B1 ELISA test kit (Crops: corn, rice, peanut, peanut butter, feed), 96 tests, quantitative

DE-100290 1 kit
EUR 781.2

Human Allergy-related Peripheral Blood Mononuclear Cells

ABC-TC4356 1 vial Ask for price
Description: Specificity of allergen of interest is verifiable, with common and rare allergies accessible. Examples of allergies include: Animal (cat, dog), Environmental (Pollen, Seasonal, Birch, Dust Mite, Rye Grass), Food (Egg, Fish, Shrimp, Milk, Peanut, Meat, Nut), Insect Bite (Bee, Wasp).

Chicken egg ovalbumin protein (ELISA Kit, antigen, allergy grade)

OVA15-N-1000 1 g
EUR 489.6

CF®568 Arachis hypogaea Lectin PNA (Peanut)

29061-1 EA
EUR 163

CF®594 Arachis hypogaea Lectin PNA (Peanut)

29062 1mg
EUR 163
Description: Lectin from peanut (Arachis hypogaea)

CF®594 Arachis hypogaea Lectin PNA (Peanut)

29062-1 EA
EUR 163

CF®488A Arachis hypogaea Lectin PNA (Peanut)

29060 1mg
EUR 163
Description: Lectin from peanut (Arachis hypogaea)

CF®488A Arachis hypogaea Lectin PNA (Peanut)

29060-1 EA
EUR 163

CF®640R Arachis hypogaea Lectin PNA (Peanut)

29063 1mg
EUR 159
Description: Lectin from peanut (Arachis hypogaea)

CF®640R Arachis hypogaea Lectin PNA (Peanut)

29063-1 EA
EUR 159

Arachis hypogaea (Peanut) Lectin (PNA) - Mitogen Solution

21511415-1 10 mL
EUR 33.86

Arachis hypogaea (Peanut) Lectin (PNA) - Mitogen Solution

21511415-2 25 mL
EUR 59.66

Cortisol Saliva

GWB-D3318B 1x96 Assays Ask for price

Mouse Anti-Crude Peanut Extract (CPE) IgE Antibody

3070 3 m g
EUR 617
Description: Allergy, Immunology

Arachis hypogaea (Peanut) Lectin (PNA) - Coated Microplates

20140008-1 10 Plate(s)
EUR 170.11

Arachis hypogaea (Peanut) Lectin (PNA) - Coated Microplates

20140008-2 20 Plate(s)
EUR 315.59

Arachis hypogaea (Peanut) Lectin (PNA) - Coated Microplates

20140008-3 5 Plate(s)
EUR 99.65

OPCA04017-1MG - Conglutin-7 Recombinant Protein (Peanut)

OPCA04017-1MG 1mg
EUR 1977

OPCA04017-20UG - Conglutin-7 Recombinant Protein (Peanut)

OPCA04017-20UG 20ug
EUR 400

OPCA04017-100UG - Conglutin-7 Recombinant Protein (Peanut)

OPCA04017-100UG 100ug
EUR 586

OPCA04016-1MG - Arachin Ahy-3 Recombinant Protein (Peanut)

OPCA04016-1MG 1mg
EUR 1977

OPCA04016-20UG - Arachin Ahy-3 Recombinant Protein (Peanut)

OPCA04016-20UG 20ug
EUR 400

OPCA04160-20UG - Arachin Ahy-3 Recombinant Protein (Peanut)

OPCA04160-20UG 20ug
EUR 449

OPCA04016-100UG - Arachin Ahy-3 Recombinant Protein (Peanut)

OPCA04016-100UG 100ug
EUR 586

OPCA04160-100UG - Arachin Ahy-3 Recombinant Protein (Peanut)

OPCA04160-100UG 100ug
EUR 668

OPCA04160-500UG - Arachin Ahy-3 Recombinant Protein (Peanut)

OPCA04160-500UG 500ug
EUR 1534

Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7)

1-CSB-YP321587AXQ
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in Yeast

Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7)

1-CSB-EP321587AXQ
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  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in E.coli

Cortisol Saliva Kit

55R-2266 1 Kit
EUR 278
Description: Cortisol Saliva ELISA Kit

Pooled Human Saliva

IRHUSL250ML each
EUR 1272
Description: Pooled Human Saliva

Pooled Human Saliva

IRHUSL50ML each
EUR 406
Description: Pooled Human Saliva

Pooled Human Saliva

IRHUSL5ML each
EUR 118
Description: Pooled Human Saliva

Cortisol ELISA (Saliva)

E22-HC9022 96T
EUR 395

Cortisol ELISA (Saliva)

GWB-AEB745 ELISA_Kits Ask for price

SALIVA COLLECTION KIT

SK-300 -
EUR 25.05

SALIVA COLLECTION KIT

SK-325 25/PK
EUR 438.9

SALIVA COLLECTION KIT

SK-350 50/PK
EUR 709.01

SALIVA COLLECTION KIT

SK-500 (FUNNEL, TUBE, INSTRUCTIONS)
EUR 25.05

SALIVA COLLECTION KIT

SK-550 500/PACK
EUR 1890.68

Mouse Anti-Crude Peanut Extract (CPE) IgG Antibody Assay Kit

3056 1 kit
EUR 404
Description: Serum, Plasma

Mouse Anti-Crude Peanut Extract (CPE) IgA Antibody Assay Kit

3061 1 kit
EUR 404
Description: Serum, Plasma

Mouse Anti-Crude Peanut Extract (CPE) IgM Antibody Assay Kit

3062 1 kit
EUR 404
Description: Serum, Plasma

Mouse Anti-Crude Peanut Extract (CPE) IgE Antibody Assay Kit

3063 1 kit
EUR 404
Description: Serum, Plasma

Direct Saliva Melatonin

EK-DSM 96
EUR 545

Mouse Anti-Crude Peanut Extract (CPE) IgG1 Antibody Assay Kit

3057 1 kit
EUR 404
Description: Serum, Plasma

Mouse Anti-Crude Peanut Extract (CPE) IgG3 Antibody Assay Kit

3060 1 kit
EUR 404
Description: Serum, Plasma

Mouse Anti-Crude Peanut Extract (CPE) IgG2a Antibody Assay Kit

3058 1 kit
EUR 404
Description: Serum, Plasma

Mouse Anti-Crude Peanut Extract (CPE) IgG2b Antibody Assay Kit

3059 1 kit
EUR 404
Description: Serum, Plasma

Progesterone Saliva ELISA

GWB-373DFD 1x96 Assays Ask for price

DHEA free in Saliva

DES6666 96
EUR 120

IgA Saliva ELISA Kit

DEIA-S216 96T
EUR 850.8
Description: Immunoenzymatic colorimetric method for determination of IgA in saliva. Salivary IgA ELISA is intended for laboratory use only.

HIV Saliva Test Strip

INV-613 one test in one individual box or pouch
EUR 6

Recombinant Aedes aegypti 37 kDa salivary gland allergen Aed a 2 (D7)

CSB-YP321587AXQ 6191 mg Ask for price

Recombinant Aedes aegypti 37 kDa salivary gland allergen Aed a 2 (D7)

CSB-EP321587AXQ 2828 mg Ask for price

Cortisol free in Saliva

DES6611 96
EUR 120

Estradiol free in Saliva

DESLV4188 96
EUR 120

Saliva DNA Isolation Kit

AG0226 Kit Ask for price
Description: Saliva DNA Isolation Kit provides a fast and simple spin column procedure for isolating high quality DNA from saliva samples collected and preserved using AcceGen's Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using AcceGen's kit is of the excellent quality, and is compatible with a number of downstream research applications including PCR, qPCR, Southern Blot analysis, sequencing and microarray analysis.

Saliva DNA Isolation Kit

K5011050 1 kit
EUR 261

Saliva DNA Collection kit

AG0245 Kit Ask for price
Description: provides Saliva DNA Self-Collection kit which is used for the collection of human DNA from saliva. It is a Simple and convenient DNA saliva collection device that stabilizes DNA long term in 2ml. This kit can be used for proven collection, stabilization and transportation of DNA from saliva.

Single Donor Human Saliva

IRHUSLS250ML each
EUR 1995
Description: Single Donor Human Saliva

Single Donor Human Saliva

IRHUSLS50ML each
EUR 681
Description: Single Donor Human Saliva

Single Donor Human Saliva

IRHUSLS5ML each
EUR 161
Description: Single Donor Human Saliva

Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7)

AP73991 1mg
EUR 2826

Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7)

AP74583 each Ask for price

Human Saliva Amylase alpha

GWB-A9E163 1000 TESTS Ask for price

SALIVA COLLECTION & PREP KIT

SK-100 1/EA
EUR 25.05

SALIVA COLLECTION & PREP KIT

SK-110 10/PACK
EUR 180.06

SALIVA COLLECTION & PREP KIT

SK-125 25/PACK
EUR 438.9

SALIVA COLLECTION & PREP KIT

SK-150 50/PACK
EUR 709.01

Testosterone free in Saliva

DES6622 96
EUR 120

Saliva DNA Purification Kit

K1468-250 250 Preps
EUR 661.2

Saliva DNA Purification Kit

K1468-50 50 Preps
EUR 381.6

SafeQ Saliva Collection Kit

MDSQ00010100 100
EUR 828

Alpha-Amylase, Human Saliva

P1455-1 each
EUR 248.4

Androstenedione free in Saliva

DESLV4780 96
EUR 155

SALIVA KIT - SMOKE FREE WORLD

SK-200 -
EUR 25.05

Progesterone free in Saliva HS

DESLV5911 96
EUR 120

ExoFACS? Kit for Saliva Exosomes

K1234-20 each
EUR 952.8

Cortisol (Saliva) Human ELISA Kit

K7434-100 each
EUR 673.2

Human Cortisol (Saliva) ELISA Kit

80957 96
EUR 310

Human (Saliva) Amylase alpha Enzyme

abx070002-1000Units 1000 Units
EUR 477.6

GWB-D3318B-96W - Cortisol Saliva

GWB-D3318B-96W 96Wells
EUR 329

Single Donor Human Pregnancy Saliva

IRHUSLSPG5ML each
EUR 267
Description: Single Donor Human Pregnancy Saliva

Single Donor Human Pediatric Saliva

IRHUSSLPED10171 each
EUR 181
Description: Single Donor Human Pediatric Saliva
The mannequin achieved precisions on the high 100 model-identified instances of 0.930 in knowledge set II, 0.960 in knowledge set III, and 0.990 in knowledge set IV. In contrast with the keyword-search method, the deep studying mannequin lowered the variety of instances for handbook evaluation by 63.8% and recognized 24.2% extra instances of confirmed allergic reactions. The mannequin highlighted vital phrases (eg, rash, hives, and Benadryl) in prediction and prolonged the record of expert-curated key phrases by means of an consideration layer.